Journal of Analytical Methods in Chemistry

Journal of Analytical Methods in Chemistry / 1998 / Article

Open Access

Volume 20 |Article ID 686107 | https://doi.org/10.1155/S1463924698000042

Amparo Galan, Anna Padros, Marta Arambarri, Silvia Martin, "Automation of a spectrophotometric method for measuring L -carnitine in human blood serum", Journal of Analytical Methods in Chemistry, vol. 20, Article ID 686107, 4 pages, 1998. https://doi.org/10.1155/S1463924698000042

Automation of a spectrophotometric method for measuring L -carnitine in human blood serum

Abstract

A spectrometric method for the determination of L-carnitine has been developed based on the reaction of the 5,5 dithiobis-(2-nitrobenzoic) acid (DTNB) and adapted to a Technicon RA-2000 automatic analyser Química Farmacéutica Bayer, S.A.). The detection limit of the method is 13.2μmol/l, with a measurement interval ranging from 30 to 320 μmoll1. Imprecision and accuracy are good even at levels close to the detection limit (coeffcient of variation of 5.4% for within-run imprecision for a concentration of 35μmol/l). A good correlation was observed between the method studied and the radiometric method. The method evaluated has suffcient analytical sensitivity to diagnose carnitine deficiencies. The short time period required for sample processing (30 samples in 40min), the simple methodology and apparatus, the ease of personnel training and the low cost of the reagents make this method a good alternative to the classical radiometric method for evaluating serum L-carnitine in clinical laboratories without radioactive installations.

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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