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Journal of Automated Methods and Management in Chemistry
Volume 24, Issue 2, Pages 51-57
http://dx.doi.org/10.1155/S146392460200010X

Analytical evaluation of two automatic methods to measure blood CK-MB mass and troponin I

1Department of Clinical Biochemistry, Hospital Universitario Germans Trias i Pujol, Badalona, Barcelona, Spain
2Coronary Care Unit, Hospital Universitario Germans Trias i Pujol, Badalona, Barcelona, Spain
3Servicio de Bioquímica Clínica, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, Badalona, Barcelona, Spain

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The lack of standardization of methods to measure cardiac markers for coronaria ischaemia, particularly troponin, led us to perform an analytical evaluation of two new immunoassays to quantify CK-MB mass and troponin I using the Dimension R×L automatic analyser. The reliability and analytical intervals of the methods were studied as well as reference values (0.010— 0.228 μg l-1 for troponin I, 0.20—3.90 mg μg l-1 for CK-MB mass) and cuto¡ values (0.77 μg l-1 for troponin I, 5 μg l-1 for CK-MB mass) established. The cutoff values were established from 37 patients with acute myocardial infarction and from 20 with unstable angina. The absence of method cross-reactivity was corroborated using myocardial, brain and skeletal muscle tissue. Both methods were highly specific and showed good reliability and practicability in the diagnosis of coronaria ischaemia after 6 h of precordial pain.