Journal of Analytical Methods in Chemistry

Journal of Analytical Methods in Chemistry / 2003 / Article

Open Access

Volume 25 |Article ID 467647 | https://doi.org/10.1155/S1463924603000142

Masahiro Okuda, Yahiro Uemura, Noriyuki Tatsumi, "Quality control material for plasma fibrinogen test produced from purified human fibrinogen", Journal of Analytical Methods in Chemistry, vol. 25, Article ID 467647, 7 pages, 2003. https://doi.org/10.1155/S1463924603000142

Quality control material for plasma fibrinogen test produced from purified human fibrinogen

Abstract

Plasma fibrinogen measurement is a routine laboratory procedure commonly performed on automated coagulation analysers. Its determination is quantitative, not quantitative. Yet, a lack of precision has been an issue for fibrinogen measurement. A control material derived from plasma comprises many proteins, inhibitors and fatty acids, any or all of which can interfere in the fibrinogen assay. This study has attempted to develop a quality control material using purified human fibrinogen and has compared measurement precision between both purified and plasma materials. Purified fibrinogen was prepared using Cohn fraction 1 and glycine precipitation. Purified fibrinogen clottability was greater than 95%, with no main plasma proteins, lipids or fibrinogen degradation products observed. Two purified control materials were lyophilized at normal (2.30 g lm1) and abnormal (1.20 g lm1) levels of fibrinogen concentration. Precision was evaluated using a liquid-type reagent, Thrombocheck Fib(L), on automated coagulation analysers. Coefficient of variation for within-run, intraday and between-day precision of the purified materials was 0.7-3.5%. In comparison, the coefficient of variation for plasma materials ranged from 1.2 to 5.3%. These results suggest that materials prepared from purified fibrinogen can be useful to laboratory quality control by improving overall precision of fibrinogen measurement and are applicable to automated coagulation analysers.

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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