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Journal of Automated Methods and Management in Chemistry
Volume 2010 (2010), Article ID 913207, 7 pages
http://dx.doi.org/10.1155/2010/913207
Research Article

Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals

1REQUIMTE, Department of Chemistry, Faculty of Pharmacy, University of Porto, Rua Anibal Cunha 164, 4050-047 Porto, Portugal
2Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, P.O. Box 96, 13400-970 Piracicaba, Brazil

Received 13 July 2010; Accepted 2 September 2010

Academic Editor: F. R. P. Rocha

Copyright © 2010 Lúcia H. M. L. M. Santos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4  𝜇 g mL-1 with a coefficient variation <3%. A sample throughput of 55 samples h-1 was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%.