Flow-Injection Preconcentration of Chloramphenicol Using Molecularly Imprinted Polymer for HPLC Determination in Environmental Samples
Figure 4
Chromatograms recorded in HPLC system with UV detection at 278 nm using as eluent water: methanol (50/50 v/v) mixture at flow rate 1 mL/min and sample injection volume 20 μL. (a) blank solution obtained for flushing MIP bed with 10 mL water, elution with 1 mL methanol, evaporation and reconstitution in 1 mL water, (b) solution obtained by flushing MIP bed with 10 mL 15 μg/L CAP solution, elution with 1 mL methanol and reconstitution in 1 mL water, (c) injection of 150 μg/L standard solution of CAP. 1: unknown signal, 2: CAP. MIP used as 25 mg bed in SPE syringe.
