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Journal of Automated Methods and Management in Chemistry
Volume 2011 (2011), Article ID 982186, 6 pages
Research Article

A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement

1Department of Chemistry, Shaanxi Institute of Education, Xi'an 710061, China
2School of Chemistry and Material Science, Shaanxi Normal University, Xi'an 710062, China
3The Vice Squad; Xi'an Municipal Public Security Bureau Yanta, Xi'an 710061, China

Received 6 October 2010; Accepted 26 November 2010

Academic Editor: Jianxiu Du

Copyright © 2011 Youjun Di et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30C using ethanol-H3PO4 and ethylenediaminetetraacetic acid disodium salt (Na2EDTA) aqueous solution (25 : 75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K3Fe(CN)6 reaction in alkaline medium. At the flow rate of 0.8 mL/min, the vardenafil retention time ( 𝑡 𝑅 ) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8 . 0 × 1 0 7 1 . 0 × 1 0 4  mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples.