Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.)

Table 1

Recovery studies of IAA, IPA, and ABA in spiked barley samples.

%	Average (𝑛=3)±SD		Average (𝑛=3)±SD		Average (𝑛=3)±SD		Average (𝑛=9)±SD
	50 ng mL^−1 = 50%		100 ng mL^−1 = 100%		150 ng mL^−1 = 150%
	Method 1	Method 2	Method 1	Method 2	Method 1	Method 2	Method 1	Method 2
IAA	56.4±3.5	96.8±2.9	59.8±3.3	97.1±1.8	63.5±3.6	99.6±2.2	59.9±3.6	97.5±1.5
IPA	65.5±2.4	97.9±2.2	60.2±3.6	96.8±2.9	64.4±2.9	99.4±2.0	63.4±2.8	98.7±1.3
ABA	68.4±3.4	95.1±3.1	72.1±4.1	99.3±2.2	67.2±3.3	100.1±2.8	69.2±2.6	98.2±2.7