Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.)

Table 2

Validation parameters.

Parameter	Results
	IAA	IPA	ABA
Linearity range, ng mL^−1	1–10000	5–10000	5–10000
Correlation coefficient	0.9997	0.9997	0.9998
Equation	𝑦=0.0236𝑥−0.0831	𝑦=0.0211𝑥−0.5677	𝑦=0.4456𝑥−14.576
LOD, ng mL^−1	1.82	5.16	5.85
LOQ, ng mL^−1	5.51	15.64	17.73
Recovery, %	97.5±1.5	98.7±1.3	98.2±2.7
Stability	Stable over 24 h at room temperature, RSD less than 2.0%