Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics

Figure 7

ClustalW [23] comparison of the sequences for kanamycin binding aptamers derived from different types of SELEX. #3_18 (gr. 7): aptamer developed by Capture-SELEX as described here. Kana18: aptamer developed by Song et al., 2011 [27] using affinity chromatography with kanamycin-immobilized sepharose beads. Fixed regions are marked with green (5′-primer binding regions), blue (3′-primer binding regions), and magenta (docking sequence). Random regions are printed in black. Red: binding region as assumed by Song et al., 2011 [27] and underlined: stem-loop forming sequence as given in [27].