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Journal of Analytical Methods in Chemistry
Volume 2012 (2012), Article ID 754650, 8 pages
Research Article

Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin

1Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Giza 11562, Egypt
2Analytical Chemistry Department, National Organization for Drug Control and Research (NODCAR), 6 Abu Hazem Street, Pyramids Avenue, P.O. Box 29, 35521, Egypt
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, 13174 Makkah, Saudi Arabia

Received 17 November 2011; Revised 12 January 2012; Accepted 20 January 2012

Academic Editor: José Benito Quintana

Copyright © 2012 Nesrin K. Ramadan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Two simple, accurate, and sensitive methods were developed for the determination of netobimin in the presence of its degradation product. Method (A) was an HPLC method, performed on C18 column using acetonitrile/methanol/0.01 M potassium dihydrogen phosphate (56 : 14 : 30 by volume) as a mobile phase with a flow rate of 0.5 mL/min. Detection was performed at 254 nm. Method (B) was a TLC method, using silica gel 60 F254 plates; the optimized mobile phase was toluene/methanol/chloroform/ammonium hydroxide (5 : 4 : 6 : 0.1 by volume). The spots were scanned densitometrically at 346 nm. Linearity ranges were 1–10 μg/mL for method (A) and 0.5–5 μg/band for method (B), and the mean percentage recoveries were 99.3±0.7% and 99.7±0.7% for methods (A) and (B), respectively. The proposed methods were found to be specific for netobimin in the presence of up to 90% of its degradation product. Statistical comparison between the results obtained by these methods and the manufacturer method was done, and no significance difference was obtained.