Spectrophotometric Determination of Mycophenolate Mofetil as Its Charge-Transfer Complexes with Two 𝜋-Acceptors

Table 1

Sensitivity and regression parameters of the proposed methods in comparison with the reported UV-spectrophotometric methods [10].

Parameter	p-CA method	DDQ method	0.1 N HCl method	Acetate buffer method
𝜆max, nm	520	580	250	250
Color stability	5 h	20 min	—	—
Linear range, μg mL^−1	40–400	6–120	10–30	10–30
Molar absorptivity (ε), L mol^-1cm^−1	1.06 × 10^3	3.87 × 10^3	NR***	NR
Sandell sensitivity*, μg cm^−2	0.4106	0.1119	NR	NR
Limit of detection (LOD), μg mL^−1	3.96	0.79	NR	NR
Limit of quantification (LOQ), μg mL^−1	11.99	2.40	NR	NR
Regression equation, 𝑌^∗∗
Intercept (a)	0.0100	0.0376	0.00316	0.01547
Slope (b)	0.0024	0.0080	0.02201	0.02168
Standard deviation of 𝑦-axis (𝑆𝑦)	0.0118	0.0416	—	—
Standard deviation of a (𝑆a)	0.0145	0.0350	—	—
Standard deviation of b (𝑆b)	5.3 × 10^−5	4.75 × 10^−4	—	—
Correlation coefficient (𝑟)	0.9995	0.9947	0.9999	0.9996

*Limit of determination as the weight in 𝜇g per mL of solution, which corresponds to an absorbance of 𝐴=0.001 measured in a cuvette of cross-sectional area 1 cm² and l = 1 cm. ^∗∗𝑌=a+b𝑋, where 𝑌 is the absorbance, 𝑋 is concentration in μg/mL, a is intercept, and b is slope. ***NR: not reported.