Table of Contents Author Guidelines Submit a Manuscript
Journal of Analytical Methods in Chemistry
Volume 2013 (2013), Article ID 140469, 8 pages
http://dx.doi.org/10.1155/2013/140469
Research Article

Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae

1Department of Biotechnology, National Institute of Technology, Warangal 506004, India
2Department of Zoology, Kakatiya University, Warangal, Andhra Pradesh 506009, India

Received 31 May 2013; Revised 31 July 2013; Accepted 12 August 2013

Academic Editor: Ravichandra Potumarthi

Copyright © 2013 Rama Raju Baadhe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. C. Y. Chang and J. D. Keasling, “Production of isoprenoid pharmaceuticals by engineered microbes,” Nature Chemical Biology, vol. 2, no. 12, pp. 674–681, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. D. K. Ro, E. M. Paradise, M. Quellet et al., “Production of the antimalarial drug precursor artemisinic acid in engineered yeast,” Nature, vol. 440, no. 7086, pp. 940–943, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. R. R. Baadhe, N. K. Mekala, S. R. Palagiri, and S. R. Parcha, “Development of petri net-based dynamic model for improved production of farnesyl pyrophosphate by integrating mevalonate and methylerythritol phosphate pathways in yeast,” Applied Biochemistry and Biotechnology, vol. 167, no. 5, pp. 1172–1182, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Nagasaki, A. Saito, A. Doi, H. Matsuno, and S. Miyano, Foundations of Systems Biology, Springer, London, UK, 1st edition, 2009.
  5. M. C. Y. Chang, R. A. Eachus, W. Trieu, D. K. Ro, and J. D. Keasling, “Engineering Escherichia coli for production of functionalized terpenoids using plant P450s,” Nature Chemical Biology, vol. 3, no. 5, pp. 274–277, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Alper, K. Miyaoku, and G. Stephanopoulos, “Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets,” Nature Biotechnology, vol. 23, no. 5, pp. 612–616, 2005. View at Publisher · View at Google Scholar · View at Scopus
  7. O. A. Carter, R. J. Peters, and R. Croteau, “Monoterpene biosynthesis pathway construction in Escherichia coli,” Phytochemistry, vol. 64, no. 2, pp. 425–433, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. B. E. Jackson, E. A. Hart-Wells, and S. P. T. Matsuda, “Metabolic engineering to produce sesquiterpenes in yeast,” Organic Letters, vol. 5, no. 10, pp. 1629–1632, 2003. View at Publisher · View at Google Scholar · View at Scopus
  9. Y. Miura, K. Kondo, H. Shimada, T. Saito, K. Nakamura, and N. Misawa, “Production of lycopene by the food yeast, Candida utilis that does not naturally synthesize carotenoid,” Biotechnology and Bioengineering, vol. 58, no. 2-3, pp. 306–308, 1998. View at Google Scholar
  10. Y. Miura, K. Kondo, T. Saito, H. Shimada, P. D. Fraser, and N. Misawa, “Production of the carotenoids lycopene, β-carotene, and astaxanthin in the food yeast Candida utilis,” Applied and Environmental Microbiology, vol. 64, no. 4, pp. 1226–1229, 1998. View at Google Scholar · View at Scopus
  11. H. Shimada, K. Kondo, P. D. Fraser, Y. Miura, T. Saito, and N. Misawa, “Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway,” Applied and Environmental Microbiology, vol. 64, no. 7, pp. 2676–2680, 1998. View at Google Scholar · View at Scopus
  12. R. R. Baadhe, N. K. Mekala, S. R. Parcha, and Y. P. Devi, “Optimization of amorphadiene production in engineered yeast by response surface methodology,” 3 Biotech, 2013. View at Publisher · View at Google Scholar
  13. L. Albertsen, Y. Chen, L. S. Bach et al., “Diversion of flux toward sesquiterpene production in Saccharomyces cerevisiae by fusion of host and heterologous enzymes,” Applied and Environmental Microbiology, vol. 77, no. 3, pp. 1033–1040, 2011. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Lamacka and J. Sajbidor, “Ergosterol determination in Saccharomyces cerevisiae. Comparison of different methods,” Biotechnology Techniques, vol. 11, no. 10, pp. 723–725, 1997. View at Google Scholar · View at Scopus
  15. M. A. Asadollahi, J. Maury, K. Moller et al., “Production of plant sesquiterpenes in Saccharomyces cerevisiae: effect of ERG9 repression on sesquiterpene biosynthesis,” Biotechnology and Bioengineering, vol. 99, no. 3, pp. 666–677, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. X. Mao, Y. Hu, C. Liang, and C. Lu, “MET3 promoter: a tightly regulated promoter and its application in construction of conditional lethal strain,” Current Microbiology, vol. 45, no. 1, pp. 37–40, 2002. View at Publisher · View at Google Scholar · View at Scopus
  17. A. Dünkler and J. Wendland, “Use of MET3 promoters for regulated gene expression in Ashbya gossypii,” Current Genetics, vol. 52, no. 1, pp. 1–10, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. R. S. Care, J. Trevethick, K. M. Binley, and P. E. Sudbery, “The MET3 promoter: a new tool for Candida albicans molecular genetics,” Molecular Microbiology, vol. 34, no. 4, pp. 792–798, 1999. View at Google Scholar · View at Scopus
  19. R. J. Conrado, J. D. Varner, and M. P. DeLisa, “Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy,” Current Opinion in Biotechnology, vol. 19, no. 5, pp. 492–499, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. K. Jorgensen, A. V. Rasmussen, M. Morant et al., “Metabolon formation and metabolic channeling in the biosynthesis of plant natural products,” Current Opinion in Plant Biology, vol. 8, no. 3, pp. 280–291, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. L. Bulow, “Characterization of an artificial bifunctional enzyme, β-galactosidase/galactokinase, prepared by gene fusion,” European Journal of Biochemistry, vol. 163, no. 3, pp. 443–448, 1987. View at Google Scholar · View at Scopus
  22. H. Carlsson, S. Ljung, and L. Bulow, “Physical and kinetic effects on introduction of various linker regions in β-galactosidase/galactose dehydrogenase fusion enzymes,” Biochimica et Biophysica Acta, vol. 1293, no. 1, pp. 154–160, 1996. View at Publisher · View at Google Scholar · View at Scopus
  23. I. Orita, N. Sakamoto, N. Kato, H. Yurimoto, and Y. Sakai, “Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase,” Applied Microbiology and Biotechnology, vol. 76, no. 2, pp. 439–445, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. H. S. Seo, Y. J. Koo, J. Y. Lim et al., “Characterization of a bifunctional enzyme fusion of trehalose-6- phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli,” Applied and Environmental Microbiology, vol. 66, no. 6, pp. 2484–2490, 2000. View at Publisher · View at Google Scholar · View at Scopus
  25. H. Carlsson, P. Ljungcrantz, L. Bulow, and K. Mosbach, “Engineering of lactose metabolism in E. coli by introducing β-galactosidase/galactokinase fusion enzymes,” Biotechnology Letters, vol. 14, no. 6, pp. 439–444, 1992. View at Publisher · View at Google Scholar · View at Scopus
  26. N. Erdeniz, U. H. Mortensen, and R. Rothstein, “Cloning-free PCR-based allele replacement methods,” Genome Research, vol. 7, no. 12, pp. 1174–1183, 1997. View at Google Scholar · View at Scopus
  27. J. Dynesen, H. P. Smits, L. Olsson, and J. Nielsen, “Carbon catabolite repression of invertase during batch cultivations of Saccharomyces cerevisiae: the role of glucose, fructose, and mannose,” Applied Microbiology and Biotechnology, vol. 50, no. 5, pp. 579–582, 1998. View at Publisher · View at Google Scholar · View at Scopus
  28. O. N. Breivik and J. L. Owades, “Spectrophotometric semimicrodetermination of ergosterol in yeast,” Agricultural and Food Chemistry, vol. 5, no. 5, pp. 360–363, 1957. View at Google Scholar · View at Scopus
  29. P. J. Westfall, D. J. Pitera, J. R. Lenihan et al., “Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 3, pp. E111–E118, 2012. View at Publisher · View at Google Scholar · View at Scopus
  30. E. T. Buurman, A. E. Blodgett, K. G. Hull, and D. Carcanague, “Pyridines and pyrimidines mediating activity against an efflux-negative strain of Candida albicans through putative inhibition of lanosterol demethylase,” Antimicrobial Agents and Chemotherapy, vol. 48, no. 1, pp. 313–318, 2004. View at Publisher · View at Google Scholar · View at Scopus
  31. J. M. Hornby, B. W. Kebaara, and K. W. Nickerson, “Farnesol biosynthesis in Candida albicans: cellular response to sterol inhibition by zaragozic acid B,” Antimicrobial Agents and Chemotherapy, vol. 47, no. 7, pp. 2366–2369, 2003. View at Publisher · View at Google Scholar · View at Scopus
  32. L. Song, “Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants,” Analytical Biochemistry, vol. 317, no. 2, pp. 180–185, 2003. View at Publisher · View at Google Scholar · View at Scopus
  33. K. Machida, T. Tanaka, Y. Yano, S. Otani, and M. Taniguchi, “Farnesol-induced growth inhibition in Saccharomyces cerevisiae by a cell cycle mechanism,” Microbiology, vol. 145, no. 2, pp. 293–299, 1999. View at Google Scholar · View at Scopus
  34. H. J. Bouwmeester, T. E. Wallaart, M. H. A. Janssen et al., “Amorpha-4,11-diene synthase catalyses the first probable step in artemisinin biosynthesis,” Phytochemistry, vol. 52, no. 5, pp. 843–854, 1999. View at Publisher · View at Google Scholar · View at Scopus
  35. G. Scalcinati, C. Knuf, S. Partow et al., “Dynamic control of gene expression in Saccharomyces cerevisiae engineered for the production of plant sesquitepene α-santalene in a fed-batch mode,” Metabolic Engineering, vol. 14, no. 2, pp. 91–103, 2012. View at Publisher · View at Google Scholar · View at Scopus