Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

Table 6

Determination of the molar absorptivity by UV spectrophotometry and HPLC.

Method	Calibration range (ng/mL)	Equation	𝑟^2	Slope (𝜀)	Mean	Relative standard deviation (%)
UV	1,000–25,000	Abs = 36,745𝑘	0.996	36,745
		Abs = 36,506𝑘	0.992	36,506
		Abs = 36,495𝑘	0.998	36,495	36,582	0.386
HPLC	1,000–25,000	Area = 37,023𝑐	0.998	37,023
		Area = 37,819𝑐	0.995	37,819
		Area = 37,379𝑐	0.993	37,379	37,407	1.065