Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs: An Application in Pharmaceutical and Human Serum

Table 1

Calibration curve data and validation parameters.

Parameter	Inference
	Phloroglucinol	Trimethylphloroglucinol
Linearity range (µg/mL)	10–200	0.01–0.2
Correlation coefficient (𝑟)	0.9999	0.9992
Regression equation (𝑦=𝑚𝑥+𝑐); Slope (𝑚)	402.66	3.12
Intercept (𝑐)	0	0
For commercial formulation
Limit of detection (LOD) (ng/mL)	10	0.01
Limit of quantification (LOQ) (ng/mL)	33	0.033
For method sensitivity
Limit of detection (LOD) (pg/mL)	10	0.01
Limit of quantification (LOQ) (pg/mL)	33	0.033