Journal of Analytical Methods in Chemistry

Research Article

Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS

The retention times, +ESI-MS/MS data (m/z), and origins of the absorbed components in rat serum after oral administration of WJSH capsule.


No.	Rt (min.) extract/plasma	Positive ions (m/z)	Origins	Identification

1	2.05/2.04	395 [M + Na]⁺, 373 [M + H]⁺	Radix et Caulis Acanthopanacis Senticosi	Syringing
2	2.71/2.73	155 [M + H]⁺, 137 [M + H-H₂O]⁺, 111 [M + H-CO₂]⁺	Radix et Caulis Acanthopanacis Senticosi, Radix Glycyrrhizae, Rhizoma Chuanxiong, Rhizoma Zingiberis Preparata	Protocatechuic acid
3	3.25/3.28	419 [M + H]⁺, 441 [M + Na]⁺, 403 [M-CH₃]⁺	Radix Glycyrrhizae	Liquiritin
4	3.43/3.40	195 [M + H]⁺, 180 [M + H-CH₃]⁺, 151 [M + H-CO₂]⁺,	Radix et Caulis Acanthopanacis Senticosi, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Glycyrrhizae	Ferulic acid
5	3.89/3.91	223 [M + H]⁺, 192 [M + H-OCH₃]⁺, 161 [M + H-OCH₃-OCH₃]⁺	Radix et Caulis Acanthopanacis Senticosi	Isofraxidine
6	4.22/4.27	355 [M + H]⁺, 377 [M + Na]⁺, 193 [M-C₉H₇O₃]⁺, 181 [M-C₇H₁₁O₅]⁺, 175 [M-C₉H₇O₃-H₂O]⁺	Radix et Caulis Acanthopanacis Senticosi, Radix Glycyrrhizae	Chlorogenic acid
7	4.44/4.46	191 [M + H]⁺, 175, 151, 137	Radix Angelicae Sinensis	Liqustilide
8	4.73/4.69	223, 205, 195	Radix Angelicae Sinensis Semen Persicae	—
9	5.10/5.13	237, 222	Radix et Caulis Acanthopanacis Senticosi	—
10	10.87/10.89	247, 232	Radix et Caulis Acanthopanacis Senticosi	—
11		194, 176	Radix Glycyrrhizae	—
12	13.30/13.27	431, 388, 370	Rhizoma Chuanxiong	—