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Journal of Analytical Methods in Chemistry
Volume 2013 (2013), Article ID 326570, 7 pages
http://dx.doi.org/10.1155/2013/326570
Research Article

Potential Protection of Coeloglossum viride var. Bracteatum Extract against Oxidative Stress in Rat Cortical Neurons

Zhe Guo,1,2,3 Rui-Yuan Pan,1,2 and Xiao-Yan Qin1,2

1Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, China
2College of Life & Environmental Science, Minzu University of China, Beijing 100081, China
3Emergency Department, The Third Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

Received 24 September 2013; Accepted 5 November 2013

Academic Editor: Yong Cheng

Copyright © 2013 Zhe Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study explored the neuroprotective effect of Coeloglossum viride var. bracteatum extract (CE) against oxidative stress in rat cortical neurons. The results demonstrated that administration of CE inhibited hydrogen peroxide-induced neurotoxicity tested by MTT, LDH release, and TUNEL assays. We further found that CE inhibited the activation of caspase-3 (Csp3) induced by hydrogen peroxide. Moreover, CE was found to reverse the hydrogen peroxide-induced downregulation of active AKT and Bcl-2. We then showed that the neuroprotective effect of CE was blocked by adding the AKT inhibitor, Ly294002. Thus, our data strongly indicated that CE played a neuroprotective role against oxidative stress-induced neurotoxicity.