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Journal of Analytical Methods in Chemistry
Volume 2013 (2013), Article ID 352606, 8 pages
http://dx.doi.org/10.1155/2013/352606
Research Article

Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers

1Centro de Pesquisas e Tecnologia em Recursos Naturais (CPTREN), Pós-Gradução em Recursos Naturais (PGRN), Departamento de Química, Universidade Estadual de Mato Grosso do Sul (UEMS), Rua Emílio Mascolli, 275, 79950-000 Naviraí, MS, Brazil
2Complexos e Centrais de Apoio a Pesquisa (COMCAP), Departamento de Química, Universidade Estadual de Maringá (UEM), Avenida Colombo, 5790, Jd Universitário, 87020-900 Maringá, PR, Brazil

Received 9 May 2013; Revised 6 August 2013; Accepted 7 August 2013

Academic Editor: Mohamed Abdel-Rehim

Copyright © 2013 Celia Eliane de Lara da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The chemical composition of essential oil and volatile obtained from the roots of Jatropha ribifolia (Pohl) Baill was performed in this work. The Clevenger extractor was utilized in hydrodistillation of oil and chemical composition determined by gas chromatography coupled with mass spectrometry detector (GC-MS). The identification of compounds was confirmed by retention index (Kovats index) obtained from a series of straight chain alkanes ( ) and by comparison with NIST and ADAMS library. A total of 61 compounds were identified in essential oil by GC-MS. The extraction of volatile was performed also by the use of the solid phase microextraction (SPME) with four different fibers. The essential oil extraction was extremely rapid (15 s) to avoid saturation of the fiber and the MS detector. The majority of the composition of essential oil is the terpenes: -pinene (major compound 9.16%), -vatirene (8.34%), -gurjunene (6.98%), -pinene (6.35%), camphene (4.34%), tricyclene (3.79%) and dehydro aromadendrene (3.52%) it and aldehydes and alcohols. Through the SPME it was possible to determine the nine volatile compounds not identified in oil 2,3,4-trimethyl-2-cyclopenten-1-one, -phellandrene, 3-carene, trans- -mentha-2,8-dienol, pinocamphone, D-verbenon, 1,3,3-trimethyl-2-(2-methyl-cyclopropyl)-cyclohexene, 2,4-diisocyanato-1-methylbenzene, and (6-hydroxymethyl-2,3-dimethylehenyl) methanol.