Journal of Analytical Methods in Chemistry / 2013 / Article / Tab 4

Research Article

Application of a Stability-Indicating HPTLC Method for Simultaneous Quantitative Determination of Olmesartan Medoxomil and Hydrochlorothiazide in Pharmaceutical Dosage Forms

Table 4

Results from robustness study.

ConditionRetention factor ( )Assaya (%)% RSD
OLMHTZOLMHTZOLMHTZ

Mobile phase composition (v/v/v/v)
 Toluene : methanol : ethyl acetate : acetone
  (2.4 : 1 : 1 : 1.5)0.310.54100.70101.200.410.69
  (2.5 : 1 : 1 : 2.5)0.320.5399.30100.761.211.08
  (2.5 : 0.9 : 0.9 : 1.5)0.310.5498.7297.910.930.86
 Development distance (cm)
  60.300.5498.76101.210.710.19
  70.310.5297.8699.300.270.87
 Time of spotting to chromatogram (min)
  90.300.5298.6199.321.321.47
  100.280.52100.86101.201.620.72
 Detection wavelength (nm)
  2750.290.5398.7199.411.010.43
  2800.290.5498.7699.370.370.86

aMean of three determinations, RSD: relative standard deviation.

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