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Journal of Analytical Methods in Chemistry
Volume 2013 (2013), Article ID 420761, 7 pages
http://dx.doi.org/10.1155/2013/420761
Research Article

Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography

1Department of Chemistry, Government Degree College, Kodur, Kadapa District, Andhra Pradesh, India
2Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan
3Electroanalytical Lab, Department of Chemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India

Received 14 May 2013; Revised 13 August 2013; Accepted 29 September 2013

Academic Editor: Mohammad A. Rashid

Copyright © 2013 Chennupalle Nageswara Reddy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulation and spiked human urine samples for the first time. The reduction potential was −0.28 V versus Ag/AgCl with a hanging mercury drop electrode in Britton-Robinson buffer as supporting electrolyte. The dependence of the intensities of currents and potentials on pH, concentration, scan rate, deposition time, and nature of the supporting electrolyte was investigated. The calibration curve was found to be linear with the following equation: , with a correlation coefficient of 0.992 ( ) over a concentration range from  M to  M. In the present investigation, the achieved limit of detection (LOD) and limit of quantization (LQD) were  M and  M; respectively. Excipients did not interfere with the determination of zanosar in pharmaceutical formulation and spiked urine samples. Precision and accuracy of the developed method were checked by recovery studies in pharmaceutical formulation and spiked human urine samples.