Research Article
Rapid Screening of Drug-Protein Binding Using High-Performance Affinity Chromatography with Columns Containing Immobilized Human Serum Albumin
Table 2
Retention time, predicted pKa, experimental PPB, and result by HSA-HPLC of test compounds
| | Compound | Retention time (min) | pKa based on ACD/labs | PPB by ultrafiltration (%) | Screening result by HSA-HPLC (%) |
| | Comp. A | 0.76 | 9.49 ± 0.12 | 78.2 | — | | Comp. B | 1.70 | 14.24 ± 0.60 | 21.6 | — | | Comp. C | 1.75 | 1.17 ± 0.50 | 51.0 | <90 | | Comp. D | 4.87 | 9.46 ± 0.60 | 95.0 | >90 | | Comp. E | >5.00 | 3.17 ± 0.10 | 99.5 | >90 | | Comp. F | 0.81 | 15.06 ± 0.46 | 97.2 | — |
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—: compound is not appropriate for the method of HSA-HPLC.
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