Journal of Analytical Methods in Chemistry / 2013 / Article / Tab 3

Research Article

Cloud-Point Extraction Combined with Liquid Chromatography for the Determination of Ergosterol, a Natural Product with Diuretic Activity, in Rat Plasma, Urine, and Faeces

Table 3

Summary of stability studies of ergosterol in rat plasma, urine, and faeces under various storage conditions.

Biological matrixAdded concentration ( g/mL)Found concentration ( g/mL)Recovery (%) RSD %

Short-term stability

0.25 99.64.1
Plasma0.75 95.26.7
2.00 95.01.9
0.25 100.84.6
Urine0.75 95.61.4
2.00 95.51.6
0.25 97.22.9
Faeces25 98.82.5
100 2.1

Three freeze-thaw cycles

0.25 104.87.8
Plasma0.75 98.44.2
2.00 96.02.1
0.25 100.44.6
Urine0.75 101.23.5
2.00 96.52.2
0.25 100.43.2
Faeces25 98.82.6
100 98.93.3

Long-term stability

0.25 100.04.6
Plasma0.75 96.42.9
2.00 97.51.8
0.25 95.24.0
Urine0.75 101.23.1
2.00 103.05.2
0.25 99.64.5
Faeces25 98.42.1
100 100.12.4

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