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Journal of Analytical Methods in Chemistry
Volume 2013 (2013), Article ID 482316, 8 pages
http://dx.doi.org/10.1155/2013/482316
Research Article

A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector

1The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China
2Faculty of Mechanical Engineering and Mechanics, Ningbo University, Ningbo 315211, China
3Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China

Received 10 August 2012; Revised 6 January 2013; Accepted 9 January 2013

Academic Editor: Antonio Ruiz Medina

Copyright © 2013 Ning Gan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. C. McGill, C. A. McMahan, A. W. Zieske et al., “Associations of coronary heart disease risk factors with the intermediate lesion of atherosclerosis in youth,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 20, no. 8, pp. 1998–2004, 2000. View at Google Scholar · View at Scopus
  2. S. Kaptoge, E. Di Angelantonio, G. Lowe et al., “C-reactive protein concentration and risk of coronary heart disease, stroke, and mortality: an individual participant meta-analysis,” The Lancet, vol. 375, no. 9709, pp. 132–140, 2010. View at Publisher · View at Google Scholar
  3. I. Swiatkiewicz, M. Kozinski, P. Magielski et al., “Usefulness of C-reactive protein as a marker of early post-infarct left ventricular systolic dysfunction,” Inflammation Research, vol. 61, no. 7, pp. 725–734, 2012. View at Publisher · View at Google Scholar
  4. R. J. Bisoendial, S. M. Boekholdt, M. Vergeer, E. S. G. Stroes, and J. J. P. Kastelein, “C-reactive protein is a mediator of cardiovascular disease,” European Heart Journal, vol. 31, no. 17, pp. 2087–2095, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Danesh and M. B. Pepys, “Editorial: C-reactive protein and coronary disease: is there a causal link?” Circulation, vol. 120, no. 21, pp. 2036–2039, 2009. View at Publisher · View at Google Scholar
  6. A. Johnson, Q. F. Song, P. K. Ferrigno, P. R. Bueno, and J. J. Davis, “Davis Sensitive affimer and antibody based impedimetric label-free assays for C-reactive protein,” Analytical Chemistry, vol. 84, no. 15, pp. 6553–6560, 2012. View at Publisher · View at Google Scholar
  7. D. Kumar and B. B. Prasad, “Multiwalled carbon nanotubes embedded molecularly imprinted polymer-modified screen printed carbon electrode for the quantitative analysis of C-reactive protein,” Sensors and Actuators B: Chemical, vol. 171-172, pp. 1141–1150, 2012. View at Publisher · View at Google Scholar
  8. K. B. Male, S. Hrapovic, and J. H. T. Luong, “Electrochemically-assisted deposition of oxidases on platinum nanoparticle/multi-walled carbon nanotube-modified electrodes,” Analyst, vol. 132, no. 12, pp. 1254–1261, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. G. Z. Sauerbrey, “Use of quartz vibration for weighing thin films on a microbalance,” Physik Journal, vol. 155, pp. 206–212, 1959. View at Publisher · View at Google Scholar
  10. X. J. Chen, Y. Y. Wang, J. J. Zhou, W. Yan, X. H. Li, and J. J. Zhu, “Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film,” Analytical Chemistry, vol. 80, pp. 2133–2140, 2008. View at Publisher · View at Google Scholar
  11. J. W. Park, S. Kurosawa, H. Aizawa, Y. Goda, M. Takai, and K. Ishihara, “Piezoelectric immunosensor for bisphenol A based on signal enhancing step with 2-methacrolyloxyethyl phosphorylcholine polymeric nanoparticle,” Analyst, vol. 131, no. 1, pp. 155–162, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. Y. Uludaǧ and I. E. Tothill, “Development of a sensitive detection method of cancer biomarkers in human serum (75%) using a quartz crystal microbalance sensor and nanoparticles amplification system,” Talanta, vol. 82, no. 1, pp. 277–282, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. T. Fu, H. Wang, G. L. Shen, and R. Q. Yu, “An amplified piezoelectric immunosensor based on amplification of enzyme-catalyzed precipitation mass,” Chemical Journal of Chinese Universities, vol. 27, no. 6, pp. 1032–1035, 2006. View at Google Scholar · View at Scopus
  14. H. Wang, J. Wang, D. Choi, Z. Tang, H. Wu, and Y. Lin, “EQCM immunoassay for phosphorylated acetylcholinesterase as a biomarker for organophosphate exposures based on selective zirconia adsorption and enzyme-catalytic precipitation,” Biosensors and Bioelectronics, vol. 24, no. 8, pp. 2377–2383, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. D. Tang, Q. Li, J. Tang, B. Su, and G. Chen, “An enzyme-free quartz crystal microbalance biosensor for sensitive glucose detection in biological fluids based on glucose/dextran displacement approach,” Analytica Chimica Acta, vol. 686, no. 1-2, pp. 144–149, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. F. Magni, Y. E. van der Burgt, C. Chinello et al., “Biomarkers discovery by peptide and protein profiling in biological fluids based on functionalized magnetic beads purification and mass spectrometry,” Blood Transfusion, vol. 8, supplement 3, pp. 92–97, 2010. View at Google Scholar
  17. J. Wang, H. Y. Han, X. C. Jiang, L. Huang, L. Chen, and N. Li, “Quantum dot-based near-infrared electrochemiluminescent immunosensor with gold nanoparticle-graphene nanosheet hybrids and silica nanospheres double-assisted signal amplification,” Analytical Chemistry, vol. 84, no. 11, pp. 4893–4899, 2012. View at Publisher · View at Google Scholar
  18. M. Hnaiein, W. M. Hassen, A. Abdelghani et al., “A conductometric immunosensor based on functionalized magnetite nanoparticles for E. coli detection,” Electrochemistry Communications, vol. 10, no. 8, pp. 1152–1154, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. X. Wang, S. Huang, Z. Shan, and W. Yang, “Preparation of Fe3O4@Au nano-composites by self-assembly technique for immobilization of glucose oxidase,” Chinese Science Bulletin, vol. 54, no. 7, pp. 1176–1181, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. C. W. Lai, Y. H. Wang, C. H. Lai et al., “Iridium-complex-functionalized Fe3O4/SiO2 core/shell nanoparticles: a facile three-in-one system in magnetic resonance imaging, luminescence imaging, and photodynamic therapy,” Small, vol. 4, no. 2, pp. 218–224, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. N. Gan, H. J. Jin, T. H. Li, and L. Zheng, “Fe3O4/Au magnetic nanoparticle amplifcation strategies for ultrasensitive electrochemical immunoassay of alfa-fetoprotein,” International Journal of Nanomedicine, vol. 6, pp. 3259–3269, 2011. View at Google Scholar
  22. V.-L. Inger and M. A. Willem, “Site-directed immobilisation of antibody fragments for detection of C-reactiveprotein,” Biosensors and Bioelectronics, vol. 21, no. 7, pp. 1141–1148, 2006. View at Publisher · View at Google Scholar
  23. G. J. Wolbink, M. C. Brouwer, S. Buysmann, I. J. M. Ten Berge, and C. E. Hack, “CRP-mediated activation of complement in vivo: assessment by measuring circulating complement-C-reactive protein complexes,” Journal of Immunology, vol. 157, no. 1, pp. 473–479, 1996. View at Google Scholar · View at Scopus
  24. I. Levan-Petit, J. Cardonna, M. Garcia et al., “Sensitive ELISA for human immunoglobulin D measurement in neonate, infant, and adult sera,” Clinical Chemistry, vol. 46, no. 6, pp. 876–878, 2000. View at Google Scholar · View at Scopus