LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

Table 2

Composition of the fraction of free phytosterols in the sea buckthorn leaves determined by HPLC-NMR.

Compound	Comparative content^a	Overall content in the leave extract^b	Retention time (min)^c
Erythrodiol	3%	<0.1%	13
Uvaol	14%	0.3%	14
Oleanolic aldehyde	4%	<0.1%	16
Unknown	3%	<0.1%	24
𝛼-amyrin	12%	0.3%	27
𝛽-amyrin	47%	1.0%	28
𝛽-sitosterol	17%	0.4%	29

aMolar ratio in the phytosterol fraction, ^bweight ratio in the extract sample, and ^cisocratic method (CDCl₃ : acetonitrile, 25 : 75).