Table of Contents Author Guidelines Submit a Manuscript
Journal of Analytical Methods in Chemistry
Volume 2013 (2013), Article ID 536490, 6 pages
Research Article

Antioxidant Activity and Volatile and Phenolic Profiles of Essential Oil and Different Extracts of Wild Mint (Mentha longifolia) from the Pakistani Flora

Institute of Chemistry, Government College University Faisalabad, Faisalabad 38000, Pakistan

Received 29 May 2013; Revised 13 August 2013; Accepted 28 August 2013

Academic Editor: Shao-Nong Chen

Copyright © 2013 Tahseen Iqbal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown Mentha longifolia (M. longifolia) were studied. The essential oil from M. longifolia aerial parts was isolated by hydrodistillation technique using Clevenger-type apparatus. The extracts were prepared with three solvents of different polarity (n-hexane, dichloromethane, and methanol) using Soxhlet extractor. Maximum extract yield was obtained with methanol (12.6 g/100 g) while the minimum with dichloromethane (3.50 g/100 g). The essential oil content was found to be 1.07 g/100 g. A total of 19 constituents were identified in the M. longifolia oil using GC/MS. The main components detected were piperitenone oxide, piperitenone, germacrene D, borneol, and β-caryophyllene. The total phenolics (TP) and total flavonoids (TF) contents of the methanol extract of M. longifolia were found to be significantly higher than dichloromethane and hexane extracts. The dichloromethane and methanol extracts exhibited excellent antioxidant activity as assessed by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, bleaching β-carotene, and inhibition of linoleic acid peroxidation assays. The essential oil and hexane extract showed comparatively weaker antioxidant and free radical scavenging activities. The results of the study have validated the medicinal and antioxidant potential of M. longifolia essential oil and extracts.