Fluorimetric Immunoassay for Multianalysis of Aflatoxins

Table 2

Analytical figures of merit of the fluorometric assay developed for analysis of AFM1.

Analytical parameters	Experimental findings
Linear range	1–50 pg/mL^∗, 6.25–25 pg/mL^#
LOD	1 pg/mL
IC50	100 pg/mL^∗, 86 pg/mL^#
R.S.D.	1.1%^∗, 0.5%^#
Adj. 𝑅^2	0.987^∗, 0.991^#
Assay sample volume	40 µL
Analysis time	180 min
Sample throughput	128 samples (in triplicate) in 180 min

*PBS; #CRM-BD282.