Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method
Table 1
Collected information and contents of the seven markers of the samples ().
Sample number
Sources
Acquisition
Contents (%)* SD
time
Lim
Evo
Rut
Q1
Q2
Q3
Q4
1
Guangxi
Mar 2007
1.756 ± 0.0282
0.224 ± 0.0042
0.308 ± 0.0049
0.591 ± 0.0115
0.245 ± 0.0019
0.153 ± 0.0003
0.137 ± 0.0017
2
Guangxi
Sep 2007
1.129 ± 0.0107
0.238 ± 0.0009
0.440 ± 0.0026
0.122 ± 0.0009
0.459 ± 0.0015
0.449 ± 0.0028
0.173 ± 0.0003
3
Guangxi
Jan 2010
2.141 ± 0.0155
0.267 ± 0.0037
0.211 ± 0.0016
0.537 ± 0.0105
0.215 ± 0.0035
0.214 ± 0.0657
0.126 ± 0.0015
4
Guangxi
Apr 2010
2.161 ± 0.0342
0.471 ± 0.0077
0.447 ± 0.0078
0.110 ± 0.0016
0.499 ± 0.0078
0.512 ± 0.0088
0.185 ± 0.0028
5
Guizhou
Nov 2006
1.747 ± 0.0288
0.078 ± 0.0008
0.157 ± 0.0030
0.571 ± 0.0046
0.100 ± 0.0019
0.163 ± 0.0020
0.113 ± 0.0016
6
Guizhou
Mar 2007
1.603 ± 0.0129
0.775 ± 0.0118
0.742 ± 0.0084
0.408 ± 0.0034
0.740 ± 0.0085
0.688 ± 0.0089
0.337 ± 0.0047
7
Guizhou
Jan 2010
1.362 ± 0.0181
0.231 ± 0.0027
0.188 ± 0.0032
0.055 ± 0.0009
0.221 ± 0.0008
0.171 ± 0.0032
0.083 ± 0.0008
8
Guizhou
Mar 2010
6.111 ± 0.0529
2.070 ± 0.0251
1.019 ± 0.0121
0.375 ± 0.0056
1.326 ± 0.0198
1.273 ± 0.0251
0.487 ± 0.0092
9
Guizhou
Jun 2010
3.212 ± 0.0536
1.189 ± 0.0058
0.818 ± 0.0043
0.250 ± 0.0013
1.000 ± 0.0067
0.811 ± 0.0029
0.333 ± 0.0021
10
Guizhou
Jul 2010
1.344 ± 0.0210
0.380 ± 0.0058
0.496 ± 0.0027
0.180 ± 0.0020
0.697 ± 0.0098
0.500 ± 0.0010
0.204 ± 0.0035
11
Hunan
Apr 2009
2.621 ± 0.0433
0.407 ± 0.0063
0.387 ± 0.0065
0.213 ± 0.0008
0.437 ± 0.0048
0.412 ± 0.0010
0.217 ± 0.0023
12
Hunan
Apr 2010
1.822 ± 0.0344
0.324 ± 0.0030
0.430 ± 0.0058
0.118 ± 0.0018
0.497 ± 0.0045
0.533 ± 0.0096
0.198 ± 0.0027
13
Jiangxi
Mar 2007
1.635 ± 0.0235
1.053 ± 0.0199
0.817 ± 0.0122
0.138 ± 0.0027
0.646 ± 0.0126
0.632 ± 0.0074
0.224 ± 0.0042
14
Jiangxi
Nov 2008
1.514 ± 0.0290
0.078 ± 0.0012
0.200 ± 0.0035
0.064 ± 0.0012
0.321 ± 0.0013
0.385 ± 0.0032
0.152 ± 0.0020
15
Jiangxi
Mar 2010
1.695 ± 0.0146
0.825 ± 0.0116
0.690 ± 0.0037
0.194 ± 0.0013
0.740 ± 0.0052
0.605 ± 0.0038
0.236 ± 0.0015
16
Shanxi
Mar 2007
1.135 ± 0.0046
0.335 ± 0.0031
0.477 ± 0.0070
0.126 ± 0.0017
0.472 ± 0.0086
0.419 ± 0.0078
0.171 ± 0.0022
17
Shanxi
Jul 2010
13.478 ± 0.2313
1.967 ± 0.0350
1.127 ± 0.0174
0.543 ± 0.0028
1.881 ± 0.0358
1.151 ± 0.0211
0.592 ± 0.0062
18
Sichuan
Mar 2010
1.624 ± 0.0028
0.235 ± 0.0007
0.386 ± 0.0021
0.104 ± 0.0016
0.409 ± 0.0068
0.454 ± 0.0037
0.174 ± 0.0026
Content (%) means the content (g) of marker in 100 g crude drug. Content (%) = [found amount (g) * 20 mL/(20 L * 0.5 g)] 100%; 20 mL is the volume of sample solution; 20 L is the injection volume, and 0.5 g is the weight of pulverized crude drug.
