Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method

Table 2

Linear regression data, LOD and LOQ of investigated compounds.

Analytes	Linear regression data^a			LOD (ng)	LOQ (ng)
	Regressive equation	𝑟	Linearrange^b(𝜇g)
Lim	𝑌=264926𝑋−79642	0.9991	0.80–16	5.962	19.873
Evo	𝑌=1119030𝑋−176315	0.9997	0.25–5.0	0.199	0.664
Rut	𝑌=57966𝑋−388805	0.9990	0.25–5.0	0.454	1.514
Q1	𝑌=2563591𝑋−59536.5	0.9999	0.15–3.0	0.809	2.696
Q2	𝑌=2605282𝑋−79116	0.9999	0.25–5.0	0.580	1.935
Q3	𝑌=1253639𝑋−56012	0.9999	0.25–5.0	1.773	5.909
Q4	𝑌=2749144𝑋−68371	0.9999	0.15–3.0	1.553	4.615

aIn the linear regression data, 𝑌 refers to the peak area, 𝑋 is the concentration, and 𝑟 is the correlation coefficient of equation.
bLinear range (𝜇g) means the content of marker in injection volume (20 μL).