Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method

Table 3

Precision, repeatability, and stability of the HPLC method for determination of the seven markers.

		Precision^a						Repeatability^b		Stability^b
Analytes	Nominal amount (𝜇g)	Intraday (𝑛=6)			Interday (𝑛=6)			Mean (%)	RSD (%)	Mean (%)	RSD (%)
		Mean (𝜇g)	RSD (%)	RE (%)^c	Mean (𝜇g)	RSD (%)	RE (%)
Lim	8.0	7.88	0.54	−1.54	7.85	1.57	−1.82	2.62	1.93	2.62	0.97
Evo	2.0	1.97	0.29	−1.49	2.03	1.85	1.68	0.40	1.94	0.40	1.34
Rut	2.0	1.96	0.23	−1.79	2.04	1.53	1.96	0.39	1.19	0.39	1.63
Q1	1.2	1.18	0.18	−1.26	1.21	0.87	1.16	0.21	1.71	0.20	1.80
Q2	2.0	1.98	0.22	−1.00	2.03	0.73	1.53	0.44	1.16	0.44	0.76
Q3	2.0	1.97	0.14	−1.49	2.04	1.48	2.02	0.41	0.87	0.41	1.05
Q4	1.2	1.19	0.21	−0.48	1.22	1.85	1.37	0.22	1.29	0.22	1.12

aTested by standard mixture solution.
bTested by sample number 11 solution.
cRE (%) is short for relative error. RE (%) = [(mean − nominal amount)/nominal amount] × 100%.