Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method
Table 4
Recovery of the extraction method for determination of the seven markers.
Analytes
Amount
RSD (%)
Recovery (%)c
Original (g)a
Add (g)b
Found (g)
13.12
10.49
23.41
1.80
98.18
Lim
13.12
13.12
26.06
0.64
98.64
13.12
15.74
28.56
1.50
98.12
2.04
1.63
3.65
0.71
98.96
Evo
2.04
2.04
4.03
1.06
98.14
2.04
2.44
4.45
0.13
99.02
1.94
1.55
3.46
0.89
98.52
Rut
1.94
1.94
3.85
0.79
98.76
1.94
2.32
4.22
1.61
98.14
1.05
0.84
1.87
1.76
97.91
Q1
1.05
1.05
2.09
0.75
98.60
1.05
1.26
2.29
0.68
98.34
2.19
1.75
3.94
0.46
100.49
Q2
2.19
2.19
4.34
1.04
98.78
2.19
2.62
4.76
0.13
98.14
2.06
1.65
3.71
1.71
100.29
Q3
2.06
2.06
4.11
1.64
99.51
2.06
2.47
4.51
1.16
99.35
1.09
0.87
1.94
1.46
98.68
Q4
1.09
1.09
2.15
0.98
98.53
1.09
1.30
2.36
1.94
97.98
Tested by sample number 11 solution. The samples added known amounts of standards at low, medium, and high levels (80%, 100%, and 120% of the known amounts, resp.). Recovery (%) = [(found − original)/added] 100%. The results indicated that the developed method was reliable and accurate for the measurement of the seven analytes.
