Rapid Enzymatic Method for Pectin Methyl Esters Determination

Figure 2

Alcohol oxidase (AOX) concentration influence on the analytical signal-reaction rate. a: AOX concentration: 2.5 U mL⁻¹  (8.8±0.2)×𝑡(s)+(198±25), 𝑟=0.99, b: AOX concentration: 2 U mL⁻¹  (8.0±0.1)×𝑡(s)+(13±1)×10, 𝑟=0.997, c: AOX concentration: 1.5 U mL⁻¹  (7.14±0.05)×𝑡(s)+(89±5), 𝑟=0.9991, d: AOX concentration: 1 U mL⁻¹  (6.12±0.02)×𝑡(s)+(79±2), 𝑟=0.9997, and e: AOX concentration: 0.5 U mL⁻¹  (3.12±0.01)×𝑡(s)+(43±1), 𝑟=0.9998. Concentration of the methanol standard: 7.6 mM. Absorbance presented in milliabsorbance (mA).