Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS⁺ System

Figure 8

Effect of five compounds on (a) cell viability and LPS-induced (b) NO production in RAW 264.7 cells. RAW 264.7 cells were pretreated with five compounds for 30 min before incubation with LPS for 24 h. (a) Cytotoxicity was evaluated by an MTT assay. (b) The culture supernatant was analyzed for nitrite production. As a control, the cells were incubated with vehicle alone. Data shows mean ± SE values of triplicate determination from independent experiments. and were calculated from comparing with LPS-stimulation value.

(a)

(b)