Determination of β-Cyano-L-alanine, γ-Glutamyl-β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography

Table 1

Chromatographic gradient conditions for the analysis of GCA, BCA, and free amino acids.

Time (min)	Eluent A^a	Eluent B^b
0	96	4
3	88	12
13	88	12
30	69	31
35	69	31
40	96	4

25 mM glacial acetic acid, 0.02% (w/v) sodium azide pH 6.0.
^bAcetonitrile.