Determination of β-Cyano-L-alanine, γ-Glutamyl-β-cyano-L-alanine, and Common Free Amino Acids in Vicia sativa (Fabaceae) Seeds by Reversed-Phase High-Performance Liquid Chromatography

Table 2

Precision, calibration parameters, and sensitivity of the determination of GCA and BCA by HPLC.

Compound	Repeatability (%)		Calibration			LOQ^d (µM)	LOD^e (µM)
	Intraday^a	Interday^b	Regression equation		Linear range^c (µM)
GCA	0.28	2.76		0.9998	0.50–200	0.50	0.15
BCA	0.31	3.08		0.9997	0.50–200	0.50	0.15

RSD of peak area based on 8 runs of a solution of the standard over 1 day.
^bRSD of peak area based on 8 runs of a solution of the standard on independent days.
^cConcentration range between the limit of quantification and the upper linear limit.
^dLimit of quantification: signal/noise ratio = 10.
^eLimit of detection: signal/noise ratio = 3.
= concentration (µM).
= peak area analyte/peak area internal standard.