Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography—Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity

Table 4

Antioxidant activity (%).

	Concentration, mg/L					Scavenging activity
	25	50	100	200	500
DPPH	29.85±0.86^a	31.75±1.41^a	40.88±1.15^b	47.63±1.93^c	71.44±1.04^d	229.19±22.34^A
ABTS	12.25±0.67^a	19.76±0.63^b	32.38±0.46^c	53.83±0.27^d	88.13±0.73^e	180.94±3.48^A
RP	0.091±0.001^a	0.108±0.002^b	0.132±0.002^c	0.178±0.001^d	0.328±0.001^e	443.60±4.01^B

Assay wavelength: 1,1-diphenyl-2-picrylhydrazyl (DPPH) = 517 nm and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) = 414 nm, and reducing power (RP) = 700 nm.
Butylated hydroxytoluene (BHT) EC₅₀; DPPH: 121.85±0.39 mg/L; ABTS: 93.85±0.43 mg/L; RP: 26.71±0.69 mg/L.
Each value represents mean ± standard deviation (SD), 𝑛=5.
a--eMeans with different superscripts in the row are significantly different at 𝑃<0.05.
AEC₅₀ (mg/L) values were calculated from the calibration curves using five different concentrations (25–500 mg/L) in quintuplicate and their data were presented as 50% scavenging activity.
BRP value (EC_0.3) was reducing activity calculated from the calibration curves using five different concentrations (25–500 mg/L) in quintuplicate.