Quantification of Neurotransmitters in Mouse Brain Tissue by Using Liquid Chromatography Coupled Electrospray Tandem Mass Spectrometry

Table 3

The analytic parameters of neurotransmitters by LC-MS/MS.

Chemical	Precursor Ion ()^a	Collision energy^b	Product ion^c	Retention time (min)
BH4	242.1	20	166.0	1.44
AACA	174.0	14	114.0	1.43
Dopamine	154.1	24	90.9	0.84
Dopamine-D4	158.1	9	141.0	0.84
Serotonin	177.0	10	160.0	1.72
Serotonin-D4	181.0	12	164.0	1.72
Norepinephrine	170.1	20	107.0	1.61
Norepinephrine-D6	176.1	20	111.0	1.60
Epinephrine	184.1	9	166.0	1.61
Epinephrine-D3	187.1	9	169.0	1.61
Glutamate	148.0	16	83.9	1.83
Glutamate-D5	153.0	16	88.0	1.82
GABA	104.0	10	86.9	1.59
GABA-D6	110.1	10	93.0	1.59

GABA, -aminobutyric acid.
The detected chemicals had the greatest responses under the positive mode: the [M + H]⁺ was used as the precursor ion.
^bThe collision energy was optimized to have the greatest product ion intensity.
^cThe product ion was used for the MRM analysis.