Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System
Table 2
Comparison of extracts efficiency by online screening HPLC-ABTS in positive and negative peak area.
Extraction solvent (%)
Compounds
(min)
Positive peak
Negative peak
Average peak area (mAU)
Standard deviation (SD±)
RSDa (%)
Yield (%)
Total extraction amount (mg)
Average peak area (mAU)
Standard deviation (SD±)
RSD (%)
100% Water
BG
54.76
12.80
2.54
19.86
1.07
0.21
6.01
0.01
0.08
WG
45.53
0.20
0.22
114.26
0.01
0.01
NDb
—
—
B
36.42
8.71
10.32
118.52
0.96
0.13
2.76
3.91
141.42
W
70.11
2.93
1.32
45.18
0.23
0.04
ND
—
—
100% Ethanol
BG
54.77
505.72
26.58
5.26
12.84
0.90
166.45
1.79
1.08
WG
45.48
468.99
24.32
5.19
11.91
1.21
ND
—
—
B
36.18
803.00
126.99
15.81
20.15
1.32
161.15
15.61
9.69
W
70.18
164.19
13.56
8.26
4.16
0.24
ND
—
—
80% Aqueous ethanol
BG
54.74
545.35
31.60
5.80
8.41
11.42
179.00
13.59
7.59
WG
45.47
1258.84
232.65
18.48
19.10
38.08
ND
—
—
B
36.60
1492.57
372.90
24.98
22.46
28.79
244.52
49.46
20.23
W
70.03
292.71
16.91
5.78
4.51
4.99
ND
—
—
70% Aqueous ethanol
BG
54.76
753.16
26.72
3.55
11.34
22.70
211.85
21.91
10.34
WG
45.50
1249.30
208.61
16.70
18.53
54.40
ND
—
—
B
36.68
1634.98
384.09
23.49
24.07
45.40
267.07
53.40
20.00
W
70.06
300.91
16.19
5.38
4.52
7.39
ND
—
—
60% Aqueous ethanol
BG
54.78
1049.88
34.23
3.26
15.12
41.46
246.00
29.69
12.07
WG
45.51
1246.60
104.08
8.35
17.78
71.14
ND
—
—
B
36.59
1710.77
194.19
11.35
24.26
62.26
272.92
56.03
20.53
W
70.05
314.99
6.94
2.20
4.55
10.13
ND
—
—
RSD: relative standard deviation; bND: not detected.
