Simultaneous Quantification of Ten Active Components in Traditional Chinese Formula Sijunzi Decoction Using a UPLC-PDA Method

Table 3

Recovery of eight components in Sijunzi (𝑛=3).

Components	Contents (ug/mL)	Quantity added; (ug/mL)	Theoretical amount; (ug/mL)	Recorded amount (ug/mL)	Recovery (%)	RSD (%)
Ginsenoside Rg1	64.83	37.5	102.33	102.42	100.25	0.65
	64.83	75	139.83	139.44	99.48	0.78
	64.83	150	214.83	210.05	96.81	0.54
Ginsenoside Rb1	48.17	37.5	85.67	85.24	98.85	0.67
	48.17	75	123.17	119.96	95.72	0.82
	48.17	150	198.17	191.81	95.76	0.87
Ginsenoside Re	61.63	18.88	80.51	80.95	102.33	0.53
	61.63	37.76	99.39	99.73	100.9	0.20
	61.63	75.53	137.16	137.13	99.96	0.26
Liquiritin	57.39	22.58	79.97	78.98	95.62	0.76
	57.39	45.15	102.54	100.78	96.1	0.87
	57.39	90.3	147.69	143.72	95.6	0.84
Liquiritigenin	47.48	21.75	69.23	69.27	100.18	0.25
	47.48	43.5	90.98	91.67	101.59	0.79
	47.48	87	134.48	136.8	102.67	0.89
Glycyrrhizic acid	111.18	55.00	166.18	164.12	96.25	0.62
	111.18	110.00	221.18	217.71	96.84	0.63
	111.18	220.00	331.18	330.52	99.7	0.36
Atractylenolide III	42.08	29.50	71.58	72.45	102.95	0.83
	42.08	59.00	101.08	100.81	99.54	0.30
	42.08	118.00	160.08	160.29	100.18	0.24
Atractylenolide I	10.50	8.93	19.43	19.37	99.33	0.21
	10.50	17.85	28.35	28.47	100.67	0.20
	10.50	37.50	48.00	46.15	95.07	0.62

Recovery (%) = (recorded amount − original amount)/spiked amount * 100%.