A Stability-Indicating HPLC-DAD Method for Determination of Stiripentol: Development, Validation, Kinetics, Structure Elucidation and Application to Commercial Dosage Form

Table 3

Robustness of the method.

Parameters changed	Variation	Retention time of STP (min)	Peak area*	Bias (%)
Temperature (°C)	23	1.81	303409	−0.31
	27	1.80	312560	2.69
Flow rate (mL/min)	0.9	1.83	308654	1.41
	1.1	1.77	314565	3.35
pH	3.9	1.79	312468	2.66
	4.0	1.78	296265	−2.66
	4.2	1.84	304648	0.092
	4.3	1.82	289216	−4.98

Peak area of 10 g/mL of STP.