Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples

Figure 3

Protein calibration curve developed using NIST BSA diluted into PBS. A series of ten dilutions (in triplicate), spanning the range 0.125–5 mg/mL, was used to prepare a calibration curve. Amide I signal strength delivered by each concentration point was fitted to a regression line.