Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples

Table 1

Results of a review of the human protein database (Uniprot Release 2012_10). In total, 10 parameters were analyzed: length of the protein (Len), average molecular weight (MW), the average molecular weight per amino acid (Ave MW each AA), the number of glutamines (nQ), the number of asparagines (nN), the number of tryptophans (nW), the theoretical extinction coefficient (EC), both on a molar basis and on a mg/mL basis, the number of amide bonds, and the mass per amide bond (MW divided by amide bonds). For each parameter, the following statistical values were calculated based on all proteins in the database: average length, the standard deviation of the length (STDEV), maximum length (Max), minimum length (Min), and the coefficient of variation (CV; STDEV/average reported in percent).

	Len	MW (Da)	Ave MW each AA (Da)	nQ	nN	nW	EC (AU/(M cm))	EC (AU mL/(mg cm))	Amide bonds	Mass/Amide (Da)
Average	558	62164.2	111.5	27	20	7	59310	1.01	604	103.6
STDEV	603	66776.0	3.4	31	24	8	65792	0.51	653	3.4
Max.	34350	3816036.9	138.3	942	1111	466	3991820	5.26	36402	166.9
Min.	4	500.6	82.6	0	0	0	0	0.00	3	65.0
CV	108%	107%	3%	118%	121%	120%	111%	51%	108%	3%

Note that each of the calculated values was determined for each protein and then the statistics were calculated for each value across all proteins.