Simultaneous Determination of Cortisol and Cortisone from Human Serum by Liquid Chromatography-Tandem Mass Spectrometry

Figure 1

MRM chromatograms obtained by the present assay method. (a) Charcoal-stripped 5% FBS (blank). (b) A blank sample spiked with the standards at the concentrations of 10 ng/mL of cortisol and 10 ng/mL of cortisone and with 20 μL of working IS. (c) A healthy subject serum with the concentrations of 25 ng/mL of cortisol and 50 ng/mL of cortisone.

(a)

(b)

(c)