Research Article
Simultaneous Determination of Cortisol and Cortisone from Human Serum by Liquid Chromatography-Tandem Mass Spectrometry
Table 1
Method validation results of the LC-MS/MS assay.
| | Concentration (ng/mL) | Intra-assay () | Interassay () | Recovery (%) | LOQ (ng/mL) | | Accuracy (%) | CV (%) | Accuracy (%) | CV (%) |
| | Cortisol | 1.0 | 95.4 | 4.6 | 97.3 | 4.5 | | 1.0 | | 5.0 | 99.2 | 2.9 | 100.4 | 2.1 | 99.0 | | 50.0 | 102.5 | 5.3 | 100.4 | 1.6 | 96.1 | | 500.0 | 96.4 | 2.7 | 97.3 | 1.5 | 94.0 |
| | Cortisone | 2.5 | 98.8 | 6.0 | 92.8 | 5.8 | | 2.5 | | 5.0 | 94.0 | 4.5 | 90.1 | 2.9 | 77.1 | | 25.0 | 92.0 | 5.6 | 89.2 | 1.9 | 81.6 | | 100.0 | 97.3 | 3.6 | 96.0 | 2.3 | 81.2 |
|
|
