Journal of Analytical Methods in Chemistry / 2014 / Article / Tab 1

Research Article

Simultaneous Determination of Cortisol and Cortisone from Human Serum by Liquid Chromatography-Tandem Mass Spectrometry

Table 1

Method validation results of the LC-MS/MS assay.

Concentration (ng/mL)Intra-assay ( )Interassay ( )Recovery (%)LOQ (ng/mL)
Accuracy (%)CV (%)Accuracy (%)CV (%)

Cortisol1.095.44.697.34.51.0
5.099.22.9100.42.199.0
50.0102.55.3100.41.696.1
500.096.42.797.31.594.0

Cortisone2.598.86.092.85.82.5
5.094.04.590.12.977.1
25.092.05.689.21.981.6
100.097.33.696.02.381.2