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Journal of Analytical Methods in Chemistry
Volume 2014 (2014), Article ID 932036, 6 pages
Research Article

Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor

1Regional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, India
2Department of Pharmacy, G.S.V.M. Medical College Kanpur, Uttar Pradesh 208002, India

Received 27 May 2013; Accepted 15 October 2013; Published 2 January 2014

Academic Editor: Shixin Deng

Copyright © 2014 Vinayak Upadhya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Effects of varying temperatures with constant pressure of solvent on extraction efficiency of two chemically different alkaloids were studied. Camptothecin (CPT) from stem of Nothapodytes nimmoniana (Grah.) Mabb. and piperine from the fruits of Piper nigrum L. were extracted using Accelerated Solvent Extractor (ASE). Three cycles of extraction for a particular sample cell at a given temperature assured complete extraction. CPT and piperine were determined and quantified by using a simple and efficient UFLC-PDA (245 and 343 nm) method. Temperature increased efficiency of extraction to yield higher amount of CPT, whereas temperature had diminutive effect on yield of piperine. Maximum yield for CPT was achieved at and for piperine at . Thus, the study determines compound specific extraction of CPT from N. nimmoniana and piperine from P. nigrum using ASE method. The present study indicates the use of this method for simple, fast, and accurate extraction of the compound of interest.