Comparability of a Three-Dimensional Structure in Biopharmaceuticals Using Spectroscopic Methods

Table 2

Thiol quantification using DTNB method. Rituximab and Etanercept free thiols under native, denaturing, and denaturing-reducing conditions using DTNB method. Uncertainty values are presented in SD (𝑛=9).

	Condition	Mabthera	RSD	Kikuzubam	RSD	𝑃
Rituximab	Native	0.0175 ± 0.010	55.0	0.0200 ± 0.012	58.4	0.6233
	Denaturing	0.602 ± 0.117	19.4	0.558 ± 0.102	18.4	0.4022
	Denaturing/reducing	31.6 ± 0.8	2.7	30.8 ± 1.5	4.7	0.1475
	Condition	Enbrel	RSD	Infinitam	RSD	𝑃
Etanercept	Native	0.0241 ± 0.015	61.5	0.0137 ± 0.005	37.6	0.0758
	Denaturing	0.447 ± 0.113	25.3	0.498 ± 0.060	12.1	0.2450
	Denaturing/reducing	56.6 ± 2.5	4.4	58.9 ± 1.4	2.4	0.0229