One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection
Table 3
The average recoveries of the fourteen investigated AAs ().
Analytes
Original (mg)
Spiked (mg)
Found (mg)
Recoverya (%)
RSD (%)
Asp
0.149
0.100
0.256
106.72
2.41
0.200
0.365
107.94
2.39
0.300
0.460
103.56
3.25
Glu
0.531
0.291
0.830
102.84
4.49
0.582
1.152
106.73
1.96
0.873
1.471
107.60
2.44
Ser
0.148
0.070
0.217
97.76
1.25
0.141
0.281
94.50
0.14
0.211
0.371
105.44
4.23
Gly
0.632
0.305
0.945
103.02
0.26
0.609
1.266
104.16
2.57
0.914
1.603
106.32
1.10
Arg
0.264
0.140
0.412
105.39
2.51
0.281
0.544
99.58
1.53
0.421
0.714
106.89
1.10
Ala
0.622
0.303
0.942
105.61
2.79
0.606
1.270
107.05
3.70
0.909
1.581
105.57
1.90
Pro
1.445
0.656
2.102
100.17
2.63
1.313
2.829
105.48
1.04
1.969
3.721
107.69
2.50
Tyr
0.136
0.079
0.219
105.27
2.95
0.159
0.291
98.17
2.71
0.238
0.384
104.35
3.80
Val
0.273
0.128
0.407
104.87
3.85
0.256
0.543
105.62
1.50
0.384
0.683
106.90
3.01
Ile
0.115
0.063
0.181
103.84
2.92
0.127
0.236
95.68
0.94
0.190
0.320
107.80
3.71
Leu
0.607
0.297
0.908
101.73
2.15
0.593
1.221
103.56
1.25
0.890
1.517
102.30
3.68
Phe
0.233
0.110
0.338
94.87
4.12
0.220
0.441
94.11
2.16
0.331
0.586
106.77
2.06
Orn
0.200
0.166
0.371
103.11
2.24
0.332
0.556
107.18
1.07
0.497
0.726
105.64
3.94
Lys
0.133
0.076
0.214
106.01
3.91
0.153
0.291
103.14
2.19
0.229
0.374
105.37
2.05
Recovery (%) = 100 × (found amount − original amount)/spiked amount; the data presented as average of three determinations.
