One Single Amino Acid for Estimation the Content of Total Free Amino Acids in Qingkailing Injection Using High-Performance Liquid Chromatography-Diode Array Detection

Table 4

Relative correction factors (RCFs) of the 13 AAs.

AAs	Relative response factors
	Method I	Method II	Method III		Method IV
	𝐹	𝐹	𝐹	bi	𝐹	Bi
Asp	0.531	0.551	0.551	−0.466	0.551	−9.425
Glu	0.492	0.501	0.501	1.498	0.501	−7.462
Ser	0.662	0.696	0.696	−1.768	0.696	−10.728
Arg	0.441	0.450	0.450	0.744	0.450	−8.215
Ala	0.896	0.891	0.891	8.858	0.891	−0.101
Pro	0.749	0.730	0.730	20.829	0.730	11.870
Tyr	0.471	0.470	0.470	1.501	0.470	−7.458
Val	0.677	0.666	0.666	4.178	0.666	−4.781
Ile	0.637	0.629	0.629	1.882	0.629	−7.077
Leu	0.646	0.633	0.633	8.324	0.633	−0.636
Phe	0.533	0.514	0.514	4.430	0.514	−4.529
Orn	0.913	0.932	0.932	1.226	0.932	−7.733
Lys	0.982	0.987	0.987	2.056	0.987	−6.904