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Journal of Analytical Methods in Chemistry
Volume 2014, Article ID 964273, 8 pages
http://dx.doi.org/10.1155/2014/964273
Research Article

Determination of Sunset Yellow and Tartrazine in Food Samples by Combining Ionic Liquid-Based Aqueous Two-Phase System with High Performance Liquid Chromatography

1Analysis and Test Centre of Jiangsu Marine Resources Development Research Institute, Lianyungang 222001, China
2College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China
3School of Chemistry and Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China

Received 13 May 2014; Revised 10 July 2014; Accepted 14 July 2014; Published 5 November 2014

Academic Editor: Verónica Pino

Copyright © 2014 Ou Sha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We proposed a simple and effective method, by coupling ionic liquid-based aqueous two-phase systems (IL-ATPSs) with high performance liquid chromatography (HPLC), for the analysis of determining tartrazine and sunset yellow in food samples. Under the optimized conditions, IL-ATPSs generated an extraction efficiency of 99% for both analytes, which could then be directly analyzed by HPLC without further treatment. Calibration plots were linear in the range of 0.01–50.0 μg/mL for both Ta and SY. The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY. This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.