Review Article
Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics
Table 1
Various sorbents used in protein SPE.
| SPE type | Material | Usage |
| Reversed-phase | C3, C4, C8 | Proteins | C18 | Peptides |
| Normal phase | ZIC-HILIC | Glycoproteins, glycopeptides | TSKgel Amide-80 | Proteins | Waters HILIC | Proteins, peptides | Cotton* | Glycopeptides, glycans after release from peptides |
| IEX | WCX, SCX | Proteins | WAX, SAX | Proteins |
| Metal-chelating | Ti, Fe | Phosphopeptides | Ga | Phosphopeptides | Cu | (Phospho)peptides |
| Affinity | Lectin | Glycans, glycopeptides | Boronic acid | Glycans, glycopeptides | Blue dye | Albumin | Protein A/G | Immunoglobulins | Heparin | IgG | RNA/DNA | Plasmids, DNA binding proteins | Purine/pyrimidine derivatives | For example, ATP/GTP using enzymes | Coenzymes | Coenzyme-dependent enzymes | Vitamins | Vitamin binding proteins | Antibodies | Proteins, peptides |
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For further information see [24] and references cited therein. *Reference [25].
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