A Stability-Indicating HPLC-DAD Method for Determination of Ferulic Acid into Microparticles: Development, Validation, Forced Degradation, and Encapsulation Efficiency

Table 2

Composition of ferulic acid-loaded and unloaded PHBV/PCL microparticles.

Composition	Formulation
	M1FA0	M1FA5	M1FA10	M1FA20	M2FA0	M2FA5	M2FA10	M2FA20
Aqueous phase
Polysorbate 80 (g)	0.25	0.25	0.25	0.25	0.25	0.25	0.25	0.25
PVA (g)	4.00	4.00	4.00	4.00	4.00	4.00	4.00	4.00
Purified water (mL)	200.0	200.0	200.0	200.0	200.0	200.0	200.0	200.0
Organic phase
Ferulic acid (g)	—	0.10	0.20	0.40	—	0.10	0.20	0.40
PHBV (system M1) (g)	2.00	1.90	1.80	1.60	—	—	—	—
PCL (system M2) (g)	—	—	—	—	2.00	1.90	1.80	1.60
Chloroform (mL)	40.0	40.0	40.0	40.0	—	—	—	—
Methylene chloride (mL)	—	—	—	—	40.0	40.0	40.0	40.0